Tomographic imagery by interference microscopy

Arnaud Dubois; Elvire Guiot; Wilfrid Schwartz; Gael Moneron; Kate Grieve; Claude Boccara

doi:10.1117/12.586727

24 March 2005 Tomographic imagery by interference microscopy

Arnaud Dubois, Elvire Guiot, Wilfrid Schwartz, Gael Moneron, Kate Grieve, Claude Boccara

Proceedings Volume 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII; (2005) https://doi.org/10.1117/12.586727
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We have developed a white-light interference microscope as an alternative technique to conventional optical coherence tomography (OCT). The experimental setup is based on a Linnik interferometer illuminated with a tungsten halogen lamp. En face tomographic images are obtained in real-time without scanning by computing the difference of two phase-opposed interferometric images recorded by a CCD camera. The short coherence length of the source yields an optical sectioning ability with 0.7 μm resolution (in water). Transverse resolution of 0.9 μm is achieved by using high numerical aperture microscope objectives. A shot-noise limited detection sensitivity of 90 dB can be reached with ~ 1 s acquisition time. High-resolution images of mouse and tadpole embryos are shown.

Citation Download Citation

Arnaud Dubois, Elvire Guiot, Wilfrid Schwartz, Gael Moneron, Kate Grieve, and Claude Boccara "Tomographic imagery by interference microscopy", Proc. SPIE 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII, (24 March 2005); https://doi.org/10.1117/12.586727

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available