Space multiplexed multiphoton microscopy by temporal focusing of ultrashort pulses

Dan Oron; Eran Tal; Yaron Silberberg

doi:10.1117/12.597276

30 March 2005 Space multiplexed multiphoton microscopy by temporal focusing of ultrashort pulses

Dan Oron, Eran Tal, Yaron Silberberg

Proceedings Volume 5700, Multiphoton Microscopy in the Biomedical Sciences V; (2005) https://doi.org/10.1117/12.597276
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. Combining temporal focusing with line-scanning microscopy results in an enhanced depth resolution, equivalent to that achieved by point scanning. Both the scanningless and the line-scanning techniques are applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers.

Citation Download Citation

Dan Oron, Eran Tal, and Yaron Silberberg "Space multiplexed multiphoton microscopy by temporal focusing of ultrashort pulses", Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); https://doi.org/10.1117/12.597276

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