A dual-detector based optical fluorescence imaging system

Jason Y. Chen; Yuhua Li; Hong Liu

doi:10.1117/12.593801

29 March 2005 A dual-detector based optical fluorescence imaging system

Jason Y. Chen, Yuhua Li, Hong Liu

Proceedings Volume 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II; (2005) https://doi.org/10.1117/12.593801
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

A dual-CCD based optical fluorescence system has been developed to facilitate ratio imaging. The system consists of two custom cameras capable of operating at 490 frames per second, with 128 by 128 detector elements, and 12 bits digitization. Tissue-simulating gel phantoms containing 5-(and-6)-carboxy seminaphthofluoresceins-1 (SNAFL-1) fluorescent dye were constructed. Using an excitation light at a wavelength of 489±11 nm and appropriate filtering, the fluorescence intensities of the dye-containing gel phantoms at different emission wavelengths were measured by the system. The dual-detector system can simultaneously collect two different intensity peaks. Gel phantoms with dye concentrations ranging from 0.00001 mg/mL to 0.1 mg/mL were used. Our results indicate that the dual-CCD fluorescence system is capable of detecting fluorescence signals at very low dye concentration (as low as 0.0001 mg/mL). To simulating real clinical situations, dye-containing gel was placed under normal gel of different thickness. Our results showed that the system can capture significant fluorescence signals of dye-containing gel under normal gel of a thickness up to 0.73 cm. The intensity ratios between different emission peaks were also measured. The system is a useful research tool in some biomedical experiments.

Citation Download Citation

Jason Y. Chen, Yuhua Li, and Hong Liu "A dual-detector based optical fluorescence imaging system", Proc. SPIE 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II, (29 March 2005); https://doi.org/10.1117/12.593801

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available