Picosecond time-gated Raman spectroscopy for transcutaneous evaluation of bone composition

Michael D. Morris; Edward R. C. Draper; Allen E. Goodship; Pavel Matousek; Michael Towrie; Anthony W. Parker; Nancy P. Camacho

doi:10.1117/12.585457

28 April 2005 Picosecond time-gated Raman spectroscopy for transcutaneous evaluation of bone composition

Michael D. Morris, Edward R. C. Draper, Allen E. Goodship, Pavel Matousek, Michael Towrie, Anthony W. Parker, Nancy P. Camacho

Author Affiliations +

Proceedings Volume 5693, Optical Tomography and Spectroscopy of Tissue VI; (2005) https://doi.org/10.1117/12.585457
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

For efficacious transcutaneous monitoring of bone mineralization and matrix quality a spatially averaged measurement is needed, often over a large area. This precludes the use of confocal microscopy. We use picosecond pulsed laser excitation and Kerr-gated time-resolved data collection techniques to obtain marker bands of bone condition whilst rejecting interfering Raman scatter from skin, tendon and other overlying tissue. Alternatively, the methodology can be used to collect signals only from these overlying tissues. In all these experiments the 1 ps pulsed laser beam is focused to approximately 1 mm diameter. Raman light is then collected at specific times following the arrival of the pulse at time delays typically from 0 to 10 ps by opening an ultrafast optical shutter based on a Kerr cell that is driven by a second synchronized laser pulse. This permits specific probing of different layers of tissue. Individual delayed spectra are co-added and the resulting correction signal is subtracted from the ungated composite spectrum or from late-arriving time-resolved spectra. We have validated this methodology using tissue from the metacarpus and radius of several strains of laboratory mice. Overlying skin, flesh and tendon was removed from metacarpus and radius of one foreleg of a mouse and the tissue used as a control. The other foreleg served as the test specimen and was prepared by shaving the hair from the tissue, leaving the skin intact. Transcutaneous time-gated Raman spectra were measured on these specimens. With an 800 nm laser spatially resolved spectroscopy with depth penetration to greater than 1 mm was easily achieved. Normal and defective bone tissue were readily distinguished.

Citation Download Citation

Michael D. Morris, Edward R. C. Draper, Allen E. Goodship, Pavel Matousek, Michael Towrie, Anthony W. Parker, and Nancy P. Camacho "Picosecond time-gated Raman spectroscopy for transcutaneous evaluation of bone composition", Proc. SPIE 5693, Optical Tomography and Spectroscopy of Tissue VI, (28 April 2005); https://doi.org/10.1117/12.585457

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