Nanoparticle-labeled DNA capture elements for detection and identification of biological agents

Johnathan L. Kiel D.V.M.; Eric A. Holwitt; Jill E. Parker; Jeevalatha Vivekananda; Veronica Franz

doi:10.1117/12.566640

29 December 2004 Nanoparticle-labeled DNA capture elements for detection and identification of biological agents

Johnathan L. Kiel D.V.M., Eric A. Holwitt, Jill E. Parker, Jeevalatha Vivekananda, Veronica Franz

Proceedings Volume 5617, Optically Based Biological and Chemical Sensing for Defence; (2004) https://doi.org/10.1117/12.566640
Event: European Symposium on Optics and Photonics for Defence and Security, 2004, London, United Kingdom

Abstract

Aptamers, synthetic DNA capture elements (DCEs), can be made chemically or in genetically engineered bacteria. DNA capture elements are artificial DNA sequences, from a random pool of sequences, selected for their specific binding to potential biological warfare or terrorism agents. These sequences were selected by an affinity method using filters to which the target agent was attached and the DNA isolated and amplified by polymerase chain reaction (PCR) in an iterative, increasingly stringent, process. The probes can then be conjugated to Quantum Dots and super paramagnetic nanoparticles. The former provide intense, bleach-resistant fluorescent detection of bioagent and the latter provide a means to collect the bioagents with a magnet. The fluorescence can be detected in a flow cytometer, in a fluorescence plate reader, or with a fluorescence microscope. To date, we have made DCEs to Bacillus anthracis spores, Shiga toxin, Venezuelan Equine Encephalitis (VEE) virus, and Francisella tularensis. DCEs can easily distinguish Bacillus anthracis from its nearest relatives, Bacillus cereus and Bacillus thuringiensis. Development of a high through-put process is currently being investigated.

Citation Download Citation

Johnathan L. Kiel D.V.M., Eric A. Holwitt, Jill E. Parker, Jeevalatha Vivekananda, and Veronica Franz "Nanoparticle-labeled DNA capture elements for detection and identification of biological agents", Proc. SPIE 5617, Optically Based Biological and Chemical Sensing for Defence, (29 December 2004); https://doi.org/10.1117/12.566640

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