Time domain transmission line dielectric probe for detection of biomolecular interactions

Qin Chen; Daniel B. Roitman; Andre Knoesen

doi:10.1117/12.580138

8 December 2004 Time domain transmission line dielectric probe for detection of biomolecular interactions

Qin Chen, Daniel B. Roitman, Andre Knoesen

Proceedings Volume 5591, Lab-on-a-Chip: Platforms, Devices, and Applications; (2004) https://doi.org/10.1117/12.580138
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

A probe was developed to detect biomolecular binding events on a substrate in the microwave regime in real time and without labels. The probe consists of a coplanar transmission line fabricated on a glass slide that can detect dielectric changes in close proximity of the substrate-liquid interface. The probe behavior was evaluated by adsorbing polyelectrolyte monolayers of alternating charge. Biomolecular detection was demonstrated by immobilizing protein A on the glass surface and detecting rabbit IgG molecules in a flow channel. The sensitivity of the probe was conservatively estimated to be ~100 pg/mm².

Citation Download Citation

Qin Chen, Daniel B. Roitman, and Andre Knoesen "Time domain transmission line dielectric probe for detection of biomolecular interactions", Proc. SPIE 5591, Lab-on-a-Chip: Platforms, Devices, and Applications, (8 December 2004); https://doi.org/10.1117/12.580138

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