Study of DNA deformation under flow using optical tweezers

Eirini Theofanidou; Tsueu Ju Su; Jochen Arlt; David T. F. Dryden; William J. Hossack; Jason Crain; Wilson C. K. Poon

doi:10.1117/12.558906

18 October 2004 Study of DNA deformation under flow using optical tweezers

Eirini Theofanidou, Tsueu Ju Su, Jochen Arlt, David T. F. Dryden, William J. Hossack, Jason Crain, Wilson C. K. Poon

Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.558906
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

The stretching and unwinding of polymers under flow is important for understanding the rheological properties of dilute polymer solutions. Scaling theory based on the blob picture of single polymer chains predicts several regimes for the overall shape of a hydrodynamically deformed macromolecule. We studied the shape of a DNA molecule stretched out by steady uniform flow at different velocities using optical trapping of single DNA molecules (tethered on polystyrene beads) and single molecule fluorescence imaging. The results show a gradual transition from a non-draining regime at low velocities to a free-draining regime at high velocities, thus verifying the predictions of the free draining-shell (F-shell) blob model.

Citation Download Citation

Eirini Theofanidou, Tsueu Ju Su, Jochen Arlt, David T. F. Dryden, William J. Hossack, Jason Crain, and Wilson C. K. Poon "Study of DNA deformation under flow using optical tweezers", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.558906

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