Influence of the condenser on sample tracking via forward scattering pattern detection

Che-Liang Tsai; Bo-Jui Chang; Kuang-Lung Huang; Long Hsu

doi:10.1117/12.559535

18 October 2004 Influence of the condenser on sample tracking via forward scattering pattern detection

Che-Liang Tsai, Bo-Jui Chang, Kuang-Lung Huang, Long Hsu

Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.559535
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

Sample tracking with a high spatial sensitivity is highly desired in force measurement with optical tweezers. However, the trick that sample tracking via forward scattering pattern detection would provide a higher sensitivity than that via regular image detection has never been investigated. In this paper, we systematically study the influences of the position and the numerical aperture of the condenser on sample tracking via forward scattering pattern detection. In our experiment, a 60X condenser is used to form the forward scattering pattern of a sample bead upon a CCD camera. As the bead is transversely shifted at a step size of 30nm by a PZT XYZ stage, we measure the magnitude of the corresponding shift of the forward scattering pattern when the 60X condenser of different angular apertures is placed at various locations along the optical axis. Our result shows that the most sensitive forward scattering pattern occurs when the condenser collimates the forward scattering light from the sample bead. We also find that the larger the numerical aperture is, the higher the sensitivity of forward scattering pattern detection will be.

Citation Download Citation

Che-Liang Tsai, Bo-Jui Chang, Kuang-Lung Huang, and Long Hsu "Influence of the condenser on sample tracking via forward scattering pattern detection", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.559535

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