Dynamic correction of aberrations in microscopic imaging systems using an artificial point source

Marcus Reicherter; Witold Górski; Tobias Haist; Wolfgang Osten

doi:10.1117/12.544115

10 September 2004 Dynamic correction of aberrations in microscopic imaging systems using an artificial point source

Marcus Reicherter, Witold Górski, Tobias Haist, Wolfgang Osten

Proceedings Volume 5462, Biophotonics Micro- and Nano-Imaging; (2004) https://doi.org/10.1117/12.544115
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

In biological micromanipulation image aberrations are introduced not only by the optical system, but also by the immersion liquid. Whereas optical system aberrations are constant and it is relatively easy to measure and correct for them, the immersion caused aberrations are variable in time and space. In this paper a method using a spherical microparticle as an artificial point source for aberration control is presented. The particle is positioned by optical tweezers at the location of the biological sample. In the experiment holographic tweezers are used. They are based on computer generated holograms, written into spatial light modulators, which create light traps for the microparticle in the object plane. The light traps can be moved without any mechanically moving parts, just by changing the hologram. The particle strongly focuses the light, therefore an artificial point source in the object space is created. The illumination light is filtered, so that only the signal corresponding to a spherical wave is analyzed by the wavefront detection system. The information about the wavefront distortion is used to dynamically correct for it. This can be done by using spatial light modulators. The method is suitable for biophotonic imaging systems, where refractive index variations in the sample plane are significant. The integration with holographic tweezers is advantageous since it offers flexibility in positioning and imaging the particles.

Citation Download Citation

Marcus Reicherter, Witold Górski, Tobias Haist, and Wolfgang Osten "Dynamic correction of aberrations in microscopic imaging systems using an artificial point source", Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, (10 September 2004); https://doi.org/10.1117/12.544115

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