Metal-enhanced fluorescent biomarkers for cardiac risk assessment

Evgenia Matveeva; Zygmunt Gryczynski; Joanna B. Malicka; Ignacy Gryczynski; Joseph R. Lakowicz

doi:10.1117/12.530091

16 June 2004 Metal-enhanced fluorescent biomarkers for cardiac risk assessment

Evgenia Matveeva, Zygmunt Gryczynski, Joanna B. Malicka, Ignacy Gryczynski, Joseph R. Lakowicz

Author Affiliations +

Proceedings Volume 5327, Plasmonics in Biology and Medicine; (2004) https://doi.org/10.1117/12.530091
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.

Citation Download Citation

Evgenia Matveeva, Zygmunt Gryczynski, Joanna B. Malicka, Ignacy Gryczynski, and Joseph R. Lakowicz "Metal-enhanced fluorescent biomarkers for cardiac risk assessment", Proc. SPIE 5327, Plasmonics in Biology and Medicine, (16 June 2004); https://doi.org/10.1117/12.530091

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