Pathological changes in Alzheimer’s brain evaluated with fluorescence emission analysis (FEA)

Alexander Christov; Todd Ottman; Paula Grammas

doi:10.1117/12.528294

13 July 2004 Pathological changes in Alzheimer’s brain evaluated with fluorescence emission analysis (FEA)

Alexander Christov, Todd Ottman, Paula Grammas

Proceedings Volume 5326, Optical Biopsy V; (2004) https://doi.org/10.1117/12.528294
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Development of AD is associated with cerebrovascular deposition of amyloid beta (Aβ) as well as a progressive increase in vasular collagen content. Both AΒ and collagen are naturally fluorescent compounds when exposed to UV light. We analyzed autofluorescence emitted from brain tissue samples and isolated brain resistance vessels harvested postmortem from patients with Alzheimer's disease (AD) and age-matched controls. Fluorescence emission, excited at 355 nm with an Nd:YAG laser, was measured using a fiber-optic based fluorescence spectroscopic system for tissue analysis. Significantly higher values of fluorescence emission intensity (P<0.001) in the spectral region from 465 to 490 nm were detected in brain resistance vessel samples from AD patients compared to the normal individuals. Results from western blot analysis showed elevated levels of type I and type III collagen, and reduced levels of type IV collagen in resistance vessels from AD patients, compared to control samples. In addition, using direct scanning of the cortical suface for fluoresxcence emission by the laser-induced fluorescence spectroscopy system we detected a significantly (P<0.05) higher level of apoptosis in AD brain tissue compared to age-matched controls. Fluorescence emission analysis (FEA) appears to be a sensitive technique for detecting structural changes in AD brain tissue.

Citation Download Citation

Alexander Christov, Todd Ottman, and Paula Grammas "Pathological changes in Alzheimer’s brain evaluated with fluorescence emission analysis (FEA)", Proc. SPIE 5326, Optical Biopsy V, (13 July 2004); https://doi.org/10.1117/12.528294

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