Nanoscale optical imaging technique

Sheng Wu; Yongchun Tang

doi:10.1117/12.553705

13 July 2004 Nanoscale optical imaging technique

Sheng Wu, Yongchun Tang

Proceedings Volume 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI; (2004) https://doi.org/10.1117/12.553705
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

The optical resolution limits the observation of fine features with size below diffraction limit, e.g. 500nm. We propose and demonstrate a technique that uses collimated laser scattering to characterize such features. We use a new illumination technique that let us image bio-molecules down to 50nm with optical microscopy. We observed vesicles with size down to 50nm and visualize the flow of such tiny molecules in the live cells. We could also characterize the detailed size and molecular weight of such particles.

Citation Download Citation

Sheng Wu and Yongchun Tang "Nanoscale optical imaging technique", Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); https://doi.org/10.1117/12.553705

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