FT-IR spectrometry utilization for determining changes in erythrocyte susceptibility to oxidative stress

Cyril Petibois; Gdrard Yves Rene Deleris

doi:10.1117/12.528908

1 July 2004 FT-IR spectrometry utilization for determining changes in erythrocyte susceptibility to oxidative stress

Cyril Petibois, Gdrard Yves Rene Deleris

Proceedings Volume 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies; (2004) https://doi.org/10.1117/12.528908
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We tested the hypothesis that FT-IR spectrometry was useful for determining oxidative stress damage on erythrocytes. Endurance-trained subjects performed a standardized endurance-training session at 75% of maximal oxygen consumption each week over 19 consecutive weeks. Capillary blood samples were taken before and after test-sessions and plasma and erythrocytes were separately analyzed using Fourier-transform infrared spectrometry. Exercise-induced change in plasma concentrations and erythrocyte IR absorptivities (vC-H_n of fatty acyl moieties, vC=O and δN-H of proteins, vP=O of phospholipids, vCOO^- of amino-acids, and vC-O of lactate) were monitored and compared to training level. First training weeks induced normalization of plasma concentration changes during exercise (unchanged for glucose, moderately increased for lactate, high increases for triglycerides, glycerol, and fatty acids) while erythrocyte phospholipids alteration remained elevated (P < 0.05). Further, training reduced the exercise-induced erythrocyte lactate content increase (vC-O; P < 0.05) and phospholipids alteration (vC-H_n and vP=O; P < 0.05) during exercise. These changes paralleled the lowering of exercise-induced hemoconcentration (P < 0.05) and plasma lactate concentration increase during exercise (P < 0.05). These correlated changes between plasma and erythrocyte parameters suggest that hemoconcentration and lactate acidosis (plasmatic and intracellular) are important factors contributing to reduce erythrocyte susceptibility to oxidative stress during chronic endurance training.

Citation Download Citation

Cyril Petibois and Gdrard Yves Rene Deleris "FT-IR spectrometry utilization for determining changes in erythrocyte susceptibility to oxidative stress", Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, (1 July 2004); https://doi.org/10.1117/12.528908

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