N-acetylcysteine and acute retinal laser lesions in the colubrid snake eye

William R. Elliott III; Heike K. Rentmeister-Bryant; Norman Barsalou; Jeremy Beer; Harry Zwick

doi:10.1117/12.530362

1 July 2004 N-acetylcysteine and acute retinal laser lesions in the colubrid snake eye

William R. Elliott III, Heike K. Rentmeister-Bryant, Norman Barsalou, Jeremy Beer, Harry Zwick

Proceedings Volume 5319, Laser Interaction with Tissue and Cells XV; (2004) https://doi.org/10.1117/12.530362
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

This study examined the role of oxidative stress and the effect of a single dose treatment with N-Acetylcysteine (NAC) on the temporal development of acute laser-induced retinal injury. We used the snake eye/Scanning Laser Ophthalmoscope (SLO) model, an in vivo, non-invasive ocular imaging technique, which has the ability to image cellular retinal detail and allows for studying morphological changes of retinal injury over time. For this study 12 corn-snakes (Elaphe g. guttata) received 5 laser exposures per eye, followed by either a single dose of the antioxidant NAC (150mg/kg, IP in sterile saline) or placebo. Laser exposures were made with a Nd: VO₄ DPSS, 532nm laser, coaxially aligned to the SLO. Shuttered pulses were 20msec x 50 mW; 1mJ each. Retinal images were taken using a Rodenstock cSLO and were digitally recorded at 1, 6, 24-hrs, and at 3-wks post-exposure. Lesions were assessed by two raters blind to the conditions of the study yielding measures of damaged area and counts of missing or damaged photoreceptors. Treated eyes showed a significant beneficial effect overall, and these results suggest that oxidative stress plays a role in laser-induced retinal injury. The use of NAC or a similar antioxidant shows promise as a therapeutic tool.

Citation Download Citation

William R. Elliott III, Heike K. Rentmeister-Bryant, Norman Barsalou, Jeremy Beer, and Harry Zwick "N-acetylcysteine and acute retinal laser lesions in the colubrid snake eye", Proc. SPIE 5319, Laser Interaction with Tissue and Cells XV, (1 July 2004); https://doi.org/10.1117/12.530362

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