Intracellular photoinduced oxidative stress by zinc phthalocyanine photosensitization: a study of the early events in real time using confocal microscopy

Eleni Alexandratou; Dido Yova; Panagiotis Handris; Dimitris Kletsas; Spyros Loukas

doi:10.1117/12.500597

16 October 2003 Intracellular photoinduced oxidative stress by zinc phthalocyanine photosensitization: a study of the early events in real time using confocal microscopy

Eleni Alexandratou, Dido Yova, Panagiotis Handris, Dimitris Kletsas, Spyros Loukas

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Proceedings Volume 5142, Therapeutic Laser Applications and Laser-Tissue Interactions; (2003) https://doi.org/10.1117/12.500597
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Oxidative stress has been implicated in several biological and pathological aspects. Reactive oxygen species (ROS) have been proposed to act as signal transduction molecules activating reactions leading to cell rescue or to cell apoptosis/necrosis. In the present study, oxidative stress was induced by photosensitization of zinc phthalocyanine (ZnPc) in human fibroblasts using a photodynamic dose that did not lead to apoptosis or necrosis. The induction of oxidative stress was performed at the microscope stage in preassigned time. The cascade of phenomena evoked was studied in real time and at the single cell level using confocal laser scanning microscopy. Using specific vital fluorescent probes, alterations induced by oxidative stress in mitochondria membrane potential, in intracellular pH and in calcium concentration were recorded. Image processing and analysis techniques were used to quantify the observed changes. Subcellular localization of the photosensitizer was studied in order to determine the primary and immediate ROS target. It was found that ZnPc is mainly localized in the mitochondria region.

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Eleni Alexandratou, Dido Yova, Panagiotis Handris, Dimitris Kletsas, and Spyros Loukas "Intracellular photoinduced oxidative stress by zinc phthalocyanine photosensitization: a study of the early events in real time using confocal microscopy", Proc. SPIE 5142, Therapeutic Laser Applications and Laser-Tissue Interactions, (16 October 2003); https://doi.org/10.1117/12.500597

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KEYWORDS

Calcium

Luminescence

Confocal microscopy

Oxygen

Cell death

Microscopes

Zinc

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