Fluorescence dynamics of human epidermis (ex vivo) and skin (in vivo)

Elena Vladimirovna Salomatina; Alexander B. Pravdin

doi:10.1117/12.518859

13 October 2003 Fluorescence dynamics of human epidermis (ex vivo) and skin (in vivo)

Elena Vladimirovna Salomatina, Alexander B. Pravdin

Proceedings Volume 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV; (2003) https://doi.org/10.1117/12.518859
Event: Saratov Fall Meeting 2002 Laser Physics and Photonics, Spectroscopy, and Molecular Modeling III; Coherent Optics of Ordered and Random Media III, 2002, Saratov, Russian Federation

Abstract

The temporal behavior of autofluorescence of human skin and epidermis under continuous UV-irradiation has been studied. Fluorescence spectra and kinetic curves of fluorescence intensity have been obtained. The fluorescence intensity recovery after dark period also has been examined. The vitiligo skin and epidermis were used for comparing their spectra with reflectance and fluorescence spectra of healthy skin. The epidermal samples were prepared using surface epidermis stripping technique. It has been concluded that fluorophores being undergone the UVA photobleaching are actually present in epidermal layer, and immediate pigment darkening does contribute, no less than a half of magnitude, to the autofluorescence decrease under continuous UVA irradiation.

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Elena Vladimirovna Salomatina and Alexander B. Pravdin "Fluorescence dynamics of human epidermis (ex vivo) and skin (in vivo)", Proc. SPIE 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV, (13 October 2003); https://doi.org/10.1117/12.518859

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