Real-time DNA biosensor using passive microfluidic structures

Layne D. Williams; Steve Blair

doi:10.1117/12.472742

17 January 2003 Real-time DNA biosensor using passive microfluidic structures

Layne D. Williams, Steve Blair

Proceedings Volume 4982, Microfluidics, BioMEMS, and Medical Microsystems; (2003) https://doi.org/10.1117/12.472742
Event: Micromachining and Microfabrication, 2003, San Jose, CA, United States

Abstract

An evanescent field waveguide DNA biosensor coupled with a microfluidic sample delivery system is the platform for our studies. By employing microfluidics our aim is to reduce sample size and scale to large arrays, thereby increasing the efficiency of the sensor. We are studying the hybridization rate in small sensing zones under flow conditions, with a capture oligonucleotide immobilized on the surface of the waveguide. We are also investigating what effect passive mixing structures placed in the sensing zone have on the hybridization kinetics of the system.

Citation Download Citation

Layne D. Williams and Steve Blair "Real-time DNA biosensor using passive microfluidic structures", Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); https://doi.org/10.1117/12.472742

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