Kinetic description of dioxygen binding to human hemoglobin on the 1-100 ns time scale

I. V. Sazanovich; Vladimir A. Galievsky; J. Karpiuk; E. P. Petrov; Jacek Waluk; Boris M. Dzhagarov

doi:10.1117/12.468915

30 May 2002 Kinetic description of dioxygen binding to human hemoglobin on the 1-100 ns time scale

I. V. Sazanovich, Vladimir A. Galievsky, J. Karpiuk, E. P. Petrov, Jacek Waluk, Boris M. Dzhagarov

Proceedings Volume 4749, ICONO 2001: Novel Trends in Nonlinear Laser Spectroscopy and Optical Diagnostics and Lasers in Chemistry, Biophysics, and Biomedicine; (2002) https://doi.org/10.1117/12.468915
Event: XVII International Conference on Coherent and Nonlinear Optics (ICONO 2001), 2001, Minsk, Belarus

Abstract

The results of nanosecond optical spectroscopy studies of hemoglobin geminate recombination on the 1-100 ns time scale are presented. Analysis of transient absorption data has shown that (i) the kinetics of O₂ recombination to hemoglobin cannot be described as a single-exponential process, and (ii) the predominant part of geminate recombination takes place on the initial stage of this process. The analysis pattern as two exponentials and background yields two processes occurring during the geminate recombination on the 1-100 ns time scale having the time constants of 1.2 +/- 0.2 ns and 16 +/- 2 ns. However, the signal-to-noise ratio of the data does not allow to make an unambiguous between several possible non-exponential decay models.

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I. V. Sazanovich, Vladimir A. Galievsky, J. Karpiuk, E. P. Petrov, Jacek Waluk, and Boris M. Dzhagarov "Kinetic description of dioxygen binding to human hemoglobin on the 1-100 ns time scale", Proc. SPIE 4749, ICONO 2001: Novel Trends in Nonlinear Laser Spectroscopy and Optical Diagnostics and Lasers in Chemistry, Biophysics, and Biomedicine, (30 May 2002); https://doi.org/10.1117/12.468915

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