Role of protein kinase C in the response of an isolated neuron to photodynamic therapy

D. E. Bragin; M. S. Kolosov; Anatoly B. Uzdensky

doi:10.1117/12.475590

16 July 2002 Role of protein kinase C in the response of an isolated neuron to photodynamic therapy

D. E. Bragin, M. S. Kolosov, Anatoly B. Uzdensky

Proceedings Volume 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III; (2002) https://doi.org/10.1117/12.475590
Event: Saratov Fall Meeting 2001, 2001, Saratov, Russian Federation

Abstract

The role of protein kinase C (PKC) in response of neuronal and glial cells to photosensitization with low concentration of sulphonated aluminium phthalocianine Photosens was investigated. Changes in neuron firing and in the structure of chromatin morphology of neuron and glial nuclei were studied using PKC activator 12-0-Tetradecanoylphorbol 13- acetate (TPA) or PKC inhibitors staurosporine, hypercinin or cheleythrine. Protein kinase C is shown to be involved in the neuronal and glial cell responses. Its activation by TPA decreased neuron lifetime, caused nucleus swelling characteristic for necrosis, strongly suppressed apoptotic death of glial cells, and induced gliosis. These effects were probably associated with PKC-induced Ca²⁺ entry into cytosol. The possible cell death mechanism in this case was nectosis. Inhibition of PK, oppositely, increased neuron lifetime and caused a moderate nucleus swelling. Cheleythrine exerted pro-apoptotic effect on the glial cells surrounding the neuron, whereas other PKC inhibitors, staurosporine and hypercini, suppressed apoptosis.

Citation Download Citation

D. E. Bragin, M. S. Kolosov, and Anatoly B. Uzdensky "Role of protein kinase C in the response of an isolated neuron to photodynamic therapy", Proc. SPIE 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III, (16 July 2002); https://doi.org/10.1117/12.475590

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