Subcellular calcium signaling in cardiac cells revealed with fast two-dimensional confocal imaging

Lothar A. Blatter M.D.; Katherine A. Sheehan; Jens Kockskaemper

doi:10.1117/12.472112

21 June 2002 Subcellular calcium signaling in cardiac cells revealed with fast two-dimensional confocal imaging

Lothar A. Blatter M.D., Katherine A. Sheehan, Jens Kockskaemper

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472112
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Excitation-contraction (e-c) coupling in atrial myocytes was studied with fast confocal microscopy and simultaneous Ca²⁺-current measurements. Cat atrial myocytes lack transverse tubules and contain junctional (j-SR) and non- junctional SR (nj-SR) which both ave ryanodine receptor Ca²⁺ release channels. I_Ca triggered Ca²⁺ release from discrete peripheral j-SR release sites, which then fused into a peripheral 'ring' of elevated (Ca²⁺)_i, followed by propagation to the center and contraction. J-SR Ca²⁺ release could be terminated instantaneously by interrupting I_Ca, whereas nj-SR Ca²⁺ release continued, once initiated, even after I_Ca and j-SR Ca²⁺ release was terminated. Resting myocytes exhibited spontaneous Ca²⁺ release events including Ca²⁺ release sites. In summary, during e-c coupling in atrial cells the elevation of (Ca²⁺)_i begins with j-SR Ca²⁺ release under the tight control of I_Ca. The peripheral increase of (Ca²⁺)_i subsequently activates adjacent nj-SR resulting in centripetal propagation of activation via CICR.

Citation Download Citation

Lothar A. Blatter M.D., Katherine A. Sheehan, and Jens Kockskaemper "Subcellular calcium signaling in cardiac cells revealed with fast two-dimensional confocal imaging", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472112

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