Selective damage of pigmented cells by means of a rapidly scanned cw laser beam

Ralf Brinkmann; Norbert Koop; Mustafa Oezdemir; Clemens Alt; Georg Schuele; Charles P. Lin; Reginald Birngruber

doi:10.1117/12.472538

27 June 2002 Selective damage of pigmented cells by means of a rapidly scanned cw laser beam

Ralf Brinkmann, Norbert Koop, Mustafa Oezdemir, Clemens Alt, Georg Schuele, Charles P. Lin, Reginald Birngruber

Proceedings Volume 4617, Laser Tissue Interaction XIII: Photochemical, Photothermal, and Photomechanical; (2002) https://doi.org/10.1117/12.472538
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Selective targeting the retinal pigment epithelium (RPE) while sparing adjacent tissue such as the photoreceptors has been demonstrated by repetitively irradiating the fundus with a train of green microsecond(s) -laser pulses. The aim of this study was to investigate selective RPE effects alternatively by means of rapidly scanning a cw-laser beam across the RPE to obtain the required microsecond(s) -illumination times. The radiation of an Ar⁺ laser (514 nm) was transmitted through a 25-micrometers core diameter fiber to a scanner unit. The fiber tip was imaged onto the object plane with a magnification of 0.75. The beam was repetitively scanned across porcine RPE samples in vitro providing an irradiation time of 1.6 microsecond(s) . Cell damage was investigated with a fluorescence viability assay.

Citation Download Citation

Ralf Brinkmann, Norbert Koop, Mustafa Oezdemir, Clemens Alt, Georg Schuele, Charles P. Lin, and Reginald Birngruber "Selective damage of pigmented cells by means of a rapidly scanned cw laser beam", Proc. SPIE 4617, Laser Tissue Interaction XIII: Photochemical, Photothermal, and Photomechanical, (27 June 2002); https://doi.org/10.1117/12.472538

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