Comparing FTIR and RAPD techniques in the typing of c. albicans in a clinical set-up

Christophe L. Sandt; Ganesh D. Sockalingum; Dominique Toubas; Dominique Aubert; Herve Lepan; Claire Lepouse; Maryse Jaussaud; Alain Leon; Jean-Michel Pinon; Michel Manfait

doi:10.1117/12.460786

27 March 2002 Comparing FTIR and RAPD techniques in the typing of C. albicans in a clinical set-up

Christophe L. Sandt, Ganesh D. Sockalingum, Dominique Toubas, Dominique Aubert, Herve Lepan, Claire Lepouse, Maryse Jaussaud, Alain Leon, Jean-Michel Pinon, Michel Manfait

Author Affiliations +

Proceedings Volume 4614, Biomedical Vibrational Spectroscopy II; (2002) https://doi.org/10.1117/12.460786
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Candida albicans is an opportunistic pathogen, generally though to be of endogenous origin, with however reported outbreaks. Epidemilogy of C. albicans has been studied so far by genotypic methods mainly, including the classical RAPD analysis. Albeit powerful, genotypic techniques are expensive, time consuming and complex to implement. FTIR spectroscopy is simple, rapid, inexpensive and an increasingly used technique for the identification of microorganisms. As a phenotypic method, it provides rapid whole cells 'fingerprinting' using few consumables and can detect very subtle differences between strains of the same species. In this study, C. albicans strains isolated from 50 patients from six hospital units were collected and studied by FTIR spectroscopy and RAPD-PCR. Discrimination of strains was computed using classification algorithms on selected features of the spectral data. Results from 10 patients, for whom iterative sampling was possible, are presented and discussed. Emphasis was laid on the reproducibility of dat for strain-level identification. FTIR analysis shows that (a) the C. albicans spectra were different from one patient to another, (b) seven patients exhibit each a homogeneous group while three patients display each two groups of strains. RAPD-PCR and FTIR analyses correlate quite well showing that FTIR spectroscopy could be a potential epidemiological tool in the control of nosocomial fungal infections.

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Christophe L. Sandt, Ganesh D. Sockalingum, Dominique Toubas, Dominique Aubert, Herve Lepan, Claire Lepouse, Maryse Jaussaud, Alain Leon, Jean-Michel Pinon, and Michel Manfait "Comparing FTIR and RAPD techniques in the typing of C. albicans in a clinical set-up", Proc. SPIE 4614, Biomedical Vibrational Spectroscopy II, (27 March 2002); https://doi.org/10.1117/12.460786

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KEYWORDS

FT-IR spectroscopy

Spectroscopy

Throat

Statistical analysis

Microorganisms

Rectum

Fungi

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