Cloning of the 16S ribosomal RNA gene of a psychrophilic bacterium from the Alaskan Fox Permafrost Tunnel

Damien Marsic; Elena V. Pikuta; Richard B. Hoover; Joseph D. Ng

doi:10.1117/12.454764

5 February 2002 Cloning of the 16S ribosomal RNA gene of a psychrophilic bacterium from the Alaskan Fox Permafrost Tunnel

Damien Marsic, Elena V. Pikuta, Richard B. Hoover, Joseph D. Ng

Author Affiliations +

Proceedings Volume 4495, Instruments, Methods, and Missions for Astrobiology IV; (2002) https://doi.org/10.1117/12.454764
Event: International Symposium on Optical Science and Technology, 2001, San Diego, CA, United States

Abstract

Extreme cold environments on Earth, such as polar regions or deep ocean harbor a variety of life forms that have developed unique molecular mechanisms that allow them not only to survive, but also to proliferate under hostile conditions. Such organisms are specially relevant to astrobiology studies because they help determine the environmental limits within which life can exist. They can also have a huge potential for biotechnological applications, because of the unique properties of their macromolecules. In this study we focused on a newly isolated bacterium from the Fox Permafrost Tunnel, FTR1, that grows anaerobically at +2 degree(s)C. We describe the molecular phylogenetic analysis of this microorganism, through the cloning, sequencing and analysis of its 16S ribosomal RNA gene. Our results suggests that FTR1 is a novel species belonging to the Carnobacterium genus.

Citation Download Citation

Damien Marsic, Elena V. Pikuta, Richard B. Hoover, and Joseph D. Ng "Cloning of the 16S ribosomal RNA gene of a psychrophilic bacterium from the Alaskan Fox Permafrost Tunnel", Proc. SPIE 4495, Instruments, Methods, and Missions for Astrobiology IV, (5 February 2002); https://doi.org/10.1117/12.454764

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available