Holographic confocal microscopy

Radim Chmelik

doi:10.1117/12.417815

7 March 2001 Holographic confocal microscopy

Radim Chmelik

Proceedings Volume 4356, 12th Czech-Slovak-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics; (2001) https://doi.org/10.1117/12.417815
Event: 12th Czech-Slovak-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 2000, Velke Losiny, Czech Republic

Abstract

Application of incoherent holography for achievement of the depth-discriminated microscopic imaging was proved. The image quality described by the coherent transfer function is comparable with that of a conventional confocal microscope; the imaging speed can be substantially higher. The observed plane is imaged as a whole, continuously in time, without any scanning system. Besides the amplitude, the phase of the confocal image is reconstructed, carrying information about phase shifts inside the specimen. The depth discrimination property can be enhanced beyond the capability of a conventional confocal microscope by the use of a broad-band illumination.

Citation Download Citation

Radim Chmelik "Holographic confocal microscopy", Proc. SPIE 4356, 12th Czech-Slovak-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, (7 March 2001); https://doi.org/10.1117/12.417815

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