Application of computerized interference microscope for monitoring oscillations of dry cell weight and morphology of living cells

Gennady G. Levin; Theodor V. Bulygin; Eugene Kalinin; Gennady N. Vishnyakov

doi:10.1117/12.426766

10 May 2001 Application of computerized interference microscope for monitoring oscillations of dry cell weight and morphology of living cells

Gennady G. Levin, Theodor V. Bulygin, Eugene Kalinin, Gennady N. Vishnyakov

Author Affiliations +

Proceedings Volume 4260, Optical Diagnostics of Living Cells IV; (2001) https://doi.org/10.1117/12.426766
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Interference microscopy technique allows implementing the quantitative analysis of a living cell, for example, to measure dry cell weight and cell density spatial distribution. The authors have created the microscope for quantitative analysis of living cells and observation of the dynamics of cell vital activity. Computer-aided processing of interferograms using phase shift technique, allows carrying out continuous (over 12 hours and more) monitoring of quantitative parameters of living cells with time interval between measurements less than one minute. RMS error for dry weight of non-living fixed test object using this microscope is about 1%. Numerous experiments on living lymphocytes and erythrocytes resulted in detecting the oscillations of dry weight. Spectral analysis of obtained time series has allowed allocating harmonics with the period from 2 hours to 15 minutes. Morphometric measurements of the same cells and cross correlation analysis of these data with oscillations of a dry mass were carried out also.

Citation Download Citation

Gennady G. Levin, Theodor V. Bulygin, Eugene Kalinin, and Gennady N. Vishnyakov "Application of computerized interference microscope for monitoring oscillations of dry cell weight and morphology of living cells", Proc. SPIE 4260, Optical Diagnostics of Living Cells IV, (10 May 2001); https://doi.org/10.1117/12.426766

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