New approach to fluorescence lifetime sensing based on molecular distributions

Olaf J. Rolinski; David J. S. Birch; Lydia J. McCartney; John C. Pickup

doi:10.1117/12.426725

10 May 2001 New approach to fluorescence lifetime sensing based on molecular distributions

Olaf J. Rolinski, David J. S. Birch, Lydia J. McCartney, John C. Pickup

Proceedings Volume 4252, Advances in Fluorescence Sensing Technology V; (2001) https://doi.org/10.1117/12.426725
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Fluorescence resonance energy transfer (FRET) from donor to acceptor molecules is one of the most powerful techniques for monitoring structure and dynamics. This is because FRET has a strong spatial dependence with angstroms resolution. This dependence includes the simplest case of a random distribution of acceptors for which an analytical solution exists for the fluorescence impulse response I(t). However, in general the acceptor distribution function p(r) is not random and a unique solution cannot be found for I(t). In many important applications of FRET eg in proteins, the simple random treatment is quite inappropriate and yet the information concerning conformation changes is preserved in p(r). One approach, which as been applied to the problem of determining p(r), is to make some assumptions as to its form eg Gaussian and then try to use this to describe I(t).

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Olaf J. Rolinski, David J. S. Birch, Lydia J. McCartney, and John C. Pickup "New approach to fluorescence lifetime sensing based on molecular distributions", Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); https://doi.org/10.1117/12.426725

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