FTIR microscopic studies on normal and H-Ras transfected cultured mouse fibroblast cells

Ahmad Salman; Jagannathan Ramesh; Nili Grossman; Ziad Hammody; Beny Cohen; Shaul Mordechai

doi:10.1117/12.409332

16 November 2000 FTIR microscopic studies on normal and H-Ras transfected cultured mouse fibroblast cells

Ahmad Salman, Jagannathan Ramesh, Nili Grossman, Ziad Hammody, Beny Cohen, Shaul Mordechai

Proceedings Volume 4161, Optical Biopsy and Tissue Optics; (2000) https://doi.org/10.1117/12.409332
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

Infrared (IR) absorption spectra are well known for their selectivity and minutiae fingerprint of molecular structure. The biochemical changes in the sub-cellular levels developing in abnormal cells, including a majority of cancer forms, manifest themselves in different optical signatures, which can be detected in infrared spectroscopy. The molecular vibrational modes which are responsible for IR absorption spectra, are characteristic of the biochemistry of the cells and their sub-cellular components. We measured the infrared absorption spectra of monolayers of cultured normal and ras gene transformed mouse fibroblasts, using microscopic infrared system (micro-FTIR) technique. The absorption for normal cells was higher than the malignant ones in the spectral range 1000 - 1500 and 2800 - 3000 cm^-1. The effect on phospholipid metabolism due to ras gene incorporation is also discussed.

Citation Download Citation

Ahmad Salman, Jagannathan Ramesh, Nili Grossman, Ziad Hammody, Beny Cohen, and Shaul Mordechai "FTIR microscopic studies on normal and H-Ras transfected cultured mouse fibroblast cells", Proc. SPIE 4161, Optical Biopsy and Tissue Optics, (16 November 2000); https://doi.org/10.1117/12.409332

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