Process of thermal destruction of glucose in consequence of sterilization in autoclave in the biopharmaceutical manufacturing

Volodymyr M. Musyanovych; Olexander I. Bilyi; Nataliia I. Gudz; Andrii V. Zdyrko

doi:10.1117/12.380516

22 March 2000 Process of thermal destruction of glucose in consequence of sterilization in autoclave in the biopharmaceutical manufacturing

Volodymyr M. Musyanovych, Olexander I. Bilyi, Nataliia I. Gudz, Andrii V. Zdyrko

Author Affiliations +

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380516
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The results of studying the process of glucose' thermal decomposition in consequence of the sterilization in the autoclave and the shelf life are considered. The research of the process was carried out for 5% glucose solution for injections before and after the sterilization in the autoclave by saturated steam at 110 degree(s)C for 30 minutes by the study of UV absorption spectra, granulometric analysis in the interval 0.2 - 25.0 mkm and pH measurements. The spectra of the glucose solutions before the sterilization were found to have no absorption bands in the range from 200 nm to 400 nm. After the sterilization two absorption bands with maximal at 229 nm and 284 nm respectively appeared. After three-month storage only one absorption band with maximum at 284 nm remains. In the report the observed spectral effects are explained.

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Volodymyr M. Musyanovych, Olexander I. Bilyi, Nataliia I. Gudz, and Andrii V. Zdyrko "Process of thermal destruction of glucose in consequence of sterilization in autoclave in the biopharmaceutical manufacturing", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380516

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KEYWORDS

Glucose

Absorption

Ultraviolet radiation

Manufacturing

Absorbance

Analytical research

Molecules

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