High-density oligonucleotide probe arrays

Glenn H. McGall; Jacqueline A. Fidenza

doi:10.1117/12.380501

22 March 2000 High-density oligonucleotide probe arrays

Glenn H. McGall, Jacqueline A. Fidenza

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380501
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

High-density DNA probe arrays offer a massively parallel approach to nucleic acid sequence analysis that will have a major impact on gene-based biomedical research and clinical diagnostics. Light-directed synthesis has enabled the large- scale manufacture of arrays containing hundreds of thousands of oligonucleotide probe sequences on a glass `chip' about 1.6 cm² in size. This method is used to produce high- density GeneChip^TM probe arrays, which are now finding widespread use in the detection and analysis of mutations and polymorphisms (`genotyping'), and in a wide range of gene expression studies. This paper will discuss methods for high-resolution photolithographic array fabrication which integrate solid-phase oligonucleotide synthesis, photochemically removable protecting groups, and lithographic techniques adapted from the microelectronics industry.

Citation Download Citation

Glenn H. McGall and Jacqueline A. Fidenza "High-density oligonucleotide probe arrays", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380501

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