Fluorescence apertureless near-field microscope: a step toward imaging information in DNA

Stephen R. Quake; T. Jason Yang; Guillaume A. Lessard; Marc Unger; Emil Kartalov

doi:10.1117/12.383349

21 April 2000 Fluorescence apertureless near-field microscope: a step toward imaging information in DNA

Stephen R. Quake, T. Jason Yang, Guillaume A. Lessard, Marc Unger, Emil Kartalov

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383349
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Single molecule imaging with optical methods has become an important tool in biophysical studies. However, when imaging molecules at room temperature using far field optics, one can only resolve molecules that are separated by a distance greater than the diffraction limit of the microscope, about 220 nanometers. Near field techniques have allowed researchers to image with resolutions on the order of 30-50 nanometers. However, there are numerous reasons to try to push the resolution limit further. One that particular concerns our group is the \notion to try to image information in DNA in order to measure sequence information. To that end, we have developed a new type of near field microscope, the fluorescence apertureless near field microscope.

Citation Download Citation

Stephen R. Quake, T. Jason Yang, Guillaume A. Lessard, Marc Unger, and Emil Kartalov "Fluorescence apertureless near-field microscope: a step toward imaging information in DNA", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383349

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