New portable time-resolved photometer for monitoring the calcium dynamics of osteoblasts under mechanical and zero-gravity stimulation

Jens Struckmeier; Jochen Tenbosch; Erk Klopp; Matthias Born; Martin R. Hofmann; David B. Jones

doi:10.1117/12.384203

27 April 2000 New portable time-resolved photometer for monitoring the calcium dynamics of osteoblasts under mechanical and zero-gravity stimulation

Jens Struckmeier, Jochen Tenbosch, Erk Klopp, Matthias Born, Martin R. Hofmann, David B. Jones

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Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384203
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We introduce a compact and portable photometric system for measurements of the calcium dynamics in cells. The photometer is designed for applications in centrifuges or in zero-gravity environment and thus extremely compact and reliable. It operates with the calcium-sensitive dye Indo-1. The excitation wavelength of 345nm is generated by frequency doubling of a laser diode. Two compact photomultiplier tubes detect the fluorescent emission. The electronics provides the sensitivity of photon counting combined with simultaneous measurement of the temperature, of air pressure, and of gravitational force. Internal data storage during the experiment is possible. A newly developed cell chamber stabilizes the cell temperature to 37.0 percent C +/- 0.1 degree C and includes a perfusion system to supply the cells with medium. The system has a modular set-up providing the possibility to change light source and detectors for investigation of other ions than calcium. Quantitative measurements of the intracellular calcium concentration are based on a comprehensive calibration of our system. First experiments show that the calcium dynamics of osteosarcoma cells stimulated by parathyroid hormone is observable.

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Jens Struckmeier, Jochen Tenbosch, Erk Klopp, Matthias Born, Martin R. Hofmann, and David B. Jones "New portable time-resolved photometer for monitoring the calcium dynamics of osteoblasts under mechanical and zero-gravity stimulation", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); https://doi.org/10.1117/12.384203

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KEYWORDS

Calcium

Bone

Photometry

Calibration

Semiconductor lasers

Light sources

Ultraviolet radiation

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