Magnetic tweezers microscope for cellular manipulation

Chen-Yuan Dong; Hayden Huang; Jason D. B. Sutin; Hyuk-Sang Kwon; George E. Cragg; R. Gilbert; Richard T. Lee; Enrico Gratton; Roger D. Kamm; Douglas A. Lauffenburger; Peter T. C. So

doi:10.1117/12.384210

27 April 2000 Magnetic tweezers microscope for cellular manipulation

Chen-Yuan Dong, Hayden Huang, Jason D. B. Sutin, Hyuk-Sang Kwon, George E. Cragg, R. Gilbert, Richard T. Lee, Enrico Gratton, Roger D. Kamm, Douglas A. Lauffenburger, Peter T. C. So

Author Affiliations +

Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384210
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We present the design of a magnetic tweezers microscope for cellular manipulation. Our design allows versatile and significant 3D stress application over a large sample region. For linear force application, forces up to 250 pN per 4.5 micrometers magnetic bead can be applied. Finite element analysis shows that variance in force level is around 10 percent within an area of 300 X 300 micrometers ². Our eight-pole design potentially allows 3D liner force application and exertion of torsional stress. Furthermore, our design allows high resolution imaging using high numerical aperture objective. Both finite element analysis of magnetic field distribution and force calibration of our design are presented. As a feasibility study, we incubated fibronectin coated 4.5 micrometers polystyrene beads with Swiss 3T3 mouse fibroblast cells. Under application around 250 pN of force per magnetic particle, we observed relative movement between attached magnetic and polystyrene beads to be on the order of 1 micrometers . Elastic, viscoelastic, and creeping responses of cell surfaces were observed. Our results are consistent with previous observations using similar magnetic techniques.

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Chen-Yuan Dong, Hayden Huang, Jason D. B. Sutin, Hyuk-Sang Kwon, George E. Cragg, R. Gilbert, Richard T. Lee, Enrico Gratton, Roger D. Kamm, Douglas A. Lauffenburger, and Peter T. C. So "Magnetic tweezers microscope for cellular manipulation", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); https://doi.org/10.1117/12.384210

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