Multiphoton up-converting phosphors for use in rapid immunoassays

R. Sam Niedbala; Timothy L. Vail; Hans Feindt; S. Li; Jarrett L. Burton

doi:10.1117/12.382032

11 April 2000 Multiphoton up-converting phosphors for use in rapid immunoassays

R. Sam Niedbala, Timothy L. Vail, Hans Feindt, S. Li, Jarrett L. Burton

Proceedings Volume 3913, In-Vitro Diagnostic Instrumentation; (2000) https://doi.org/10.1117/12.382032
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

There exists increasing demand for rapid in-vitro diagnostic test capable of detecting pathogens, disease markers, and drugs. Many currently marketed technologies are limiting due to their analytical sensitivity or the number of analytes detected in a single assay. Presented is the use of up- converting phosphors as reporters in rapid point-of-care diagnostic assays using a lateral flow format in a nitrocellulose matrix. Up-converting Phosphor Technology (UPT) utilizes lanthanide-doped ceramic particles that possess anti-Stokes shift emission. These material absorb multiple IR photons and emit dopant-dependent spectra in the visible range. Their signature spectra allow the development of sensitive, multi-analyte diagnostic test. Because no biological matrix up-converts in the IR, the sensitivity of the assay is enhanced, being determined by the affinity of the biological constituents and the level of non-specific binding of the particle reporter. Feasibility data presented below represent a wide range of molecular weight analytes, including drugs of abuse, proteins such as cardiac markers, and pathogenic microorganisms.

Citation Download Citation

R. Sam Niedbala, Timothy L. Vail, Hans Feindt, S. Li, and Jarrett L. Burton "Multiphoton up-converting phosphors for use in rapid immunoassays", Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); https://doi.org/10.1117/12.382032

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available