Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping

Cristina R. Rusu; Ronny van't Oever; Meint J. de Boer; Henri V. Jansen; Erwin Berenschot; Miko C. Elwenspoek; Martin L. Bennink; Johannes Sake Kanger; Bart G. de Grooth; Jan Greve; Juergen P. Brugger; Albert van den Berg

doi:10.1117/12.379579

15 March 2000 Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping

Cristina R. Rusu, Ronny van't Oever, Meint J. de Boer, Henri V. Jansen, Erwin Berenschot, Miko C. Elwenspoek, Martin L. Bennink, Johannes Sake Kanger, Bart G. de Grooth, Jan Greve, Juergen P. Brugger, Albert van den Berg

Author Affiliations +

Proceedings Volume 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications; (2000) https://doi.org/10.1117/12.379579
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We have developed a micromachined flow cell consisting of a flow channel integrated with micropipettes. The flow cell is used in combination with an optical trap set-up (optical tweezers) to study mechanical and structural properties of (lambda) -DNA molecules. The flow cell was realized using silicon micromachining including the so-called buried channel technology to fabricate the micropipettes, the wet etching of glass to create the flow channel, and the powder blasting of glass to create the fluid connections. The volume of the flow cell is 2 (mu) l. The pipettes have a length of 130 micrometer, a width of 5 - 10 micrometer, a round opening of 1 micron and can be processed with different shapes. Using this flow cell we stretched single molecules ((lambda) -DNA) showing typical force-extension curves also found with conventional techniques.

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Cristina R. Rusu, Ronny van't Oever, Meint J. de Boer, Henri V. Jansen, Erwin Berenschot, Miko C. Elwenspoek, Martin L. Bennink, Johannes Sake Kanger, Bart G. de Grooth, Jan Greve, Juergen P. Brugger, and Albert van den Berg "Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping", Proc. SPIE 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications, (15 March 2000); https://doi.org/10.1117/12.379579

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