Preliminary findings using dynamic light scattering to study vitreous effects of pharmacologic vitreolysis

Jerry Sebag M.D.; N. Kitaya; Rafat R. Ansari; H. L. Karagoezian; Akitoshi Yoshida

doi:10.1117/12.387525

7 June 2000 Preliminary findings using dynamic light scattering to study vitreous effects of pharmacologic vitreolysis

Jerry Sebag M.D., N. Kitaya, Rafat R. Ansari, H. L. Karagoezian, Akitoshi Yoshida

Proceedings Volume 3908, Ophthalmic Technologies X; (2000) https://doi.org/10.1117/12.387525
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Pharmacologic Vitreolysis is a new therapy intended to enzymatically liquefy vitreous and separate the posterior vitreous cortex from the retina. There are presently no non- invasive methods with which to quantify vitreous liquefaction. This study evaluated non-invasive Dynamic Light Scattering (DLS) to assess vitreous liquefaction in model vitreous solutions. Vitreous of 5 bovine eyes was measured by DLS. Methodology involved the addition 50 IU/ml of hyaluronidase and 5 mg/ml of collagenase to solutions of 0.1 mg/ml of hyaluronan and collagen with polystyrene beads (30 nm diameter), respectively. Solutions were incubated at 37 degrees Celsius for 24 hours and DLS measurements were made at various time intervals. Results showed that the diffusion coefficients and particle size distributions determined from DLS measurements in the 5 bovine vitreous specimens were consistent and reproducible. Adding enzyme to the model vitreous solutions increased diffusion coefficients in the collagen as well as the HA solutions. These results suggest that DLS is a useful non-invasive method to measure diffusion coefficients in both model solutions and bovine vitreous. This approach may provide a quantitative means to assess different pharmacologic vitreolysis agents in vitro, and may also serve as a clinical tool for monitoring the effects of pharmacologic vitreolysis in vivo.

Citation Download Citation

Jerry Sebag M.D., N. Kitaya, Rafat R. Ansari, H. L. Karagoezian, and Akitoshi Yoshida "Preliminary findings using dynamic light scattering to study vitreous effects of pharmacologic vitreolysis", Proc. SPIE 3908, Ophthalmic Technologies X, (7 June 2000); https://doi.org/10.1117/12.387525

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available