Observation of DNA fixed on a sapphire substrate by a collection mode near-field optical microscope

Uma Maheswari Rajagopalan; Shuji Mononobe; Kenji Yoshida; Mamoru Yoshimoto; Motoichi Ohtsu

doi:10.1117/12.346822

29 April 1999 Observation of DNA fixed on a sapphire substrate by a collection mode near-field optical microscope

Uma Maheswari Rajagopalan, Shuji Mononobe, Kenji Yoshida, Mamoru Yoshimoto, Motoichi Ohtsu

Author Affiliations +

Proceedings Volume 3729, Selected Papers from International Conference on Optics and Optoelectronics '98; (1999) https://doi.org/10.1117/12.346822
Event: Selected Papers from the International Conference on Optics and Optoelectronics, 1998, Dehradun, India

Abstract

We present the observation results of a single string DNA (deoxy ribo nucleic acid) obtained by collection mode near- field optical microscopy operated under constant distance mode with the optical near field intensity as the feedback signal. The observed width of the narrowest string is 4 nm. To our knowledge, this is not only the successful optical observation of a single string DNA by purely optical means but also under such high resolving capability. These are attributed to the special care taken during the sample preparation to avoid unwanted scattered light and to the probe which were specially designed to enhance the local near field interaction. All these factors make the probe picks up efficiently the high spatial frequency components of the scattered near field at the same time rejecting effectively the lower spatial frequency components.

Citation Download Citation

Uma Maheswari Rajagopalan, Shuji Mononobe, Kenji Yoshida, Mamoru Yoshimoto, and Motoichi Ohtsu "Observation of DNA fixed on a sapphire substrate by a collection mode near-field optical microscope", Proc. SPIE 3729, Selected Papers from International Conference on Optics and Optoelectronics '98, (29 April 1999); https://doi.org/10.1117/12.346822

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