Semiautomatic quality determination of 3D confocal microscope scans of neuronal cells denoised by 3D wavelet shrinkage

Anca Dima; Michael Scholz; Klaus Obermayer

doi:10.1117/12.342957

22 March 1999 Semiautomatic quality determination of 3D confocal microscope scans of neuronal cells denoised by 3D wavelet shrinkage

Anca Dima, Michael Scholz, Klaus Obermayer

Author Affiliations +

Proceedings Volume 3723, Wavelet Applications VI; (1999) https://doi.org/10.1117/12.342957
Event: AeroSense '99, 1999, Orlando, FL, United States

Abstract

The main goal of this work is to denoise 3D confocal microscope scans of neuronal cells taken at high resolution such that neuronal structures of size smaller than 1 micrometers become visible. Although scanning confocal microscopes give much clearer images than ordinary light microscopes do, the images are still noisy and blurred. Our goal is to filter out the noise in these images without disturbing the smallest neuronal structures which have the same signal amplitude and geometric size as the noise. In order to obtain a good scale-space representation of the analyzed image, we use the 3D-wavelet transformation. We extend the denoising method of Donoho for 3D data and obtain several ways of computing thresholds and noise variances. Finally we develop a quality measure, for images with tree like structures, to determine the denoising method and wavelet form best suited for a particular confocal scan.

Citation Download Citation

Anca Dima, Michael Scholz, and Klaus Obermayer "Semiautomatic quality determination of 3D confocal microscope scans of neuronal cells denoised by 3D wavelet shrinkage", Proc. SPIE 3723, Wavelet Applications VI, (22 March 1999); https://doi.org/10.1117/12.342957

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available