Raman imaging of bone mineral and matrix: composition and function

Angela Carden; Jerilyn A. Timlin; Clare M. Edwards; Michael D. Morris; C. Edward Hoffler; Kenneth Kozloff; Steven A. Goldstein

doi:10.1117/12.345394

9 April 1999 Raman imaging of bone mineral and matrix: composition and function

Angela Carden, Jerilyn A. Timlin, Clare M. Edwards, Michael D. Morris, C. Edward Hoffler, Kenneth Kozloff, Steven A. Goldstein

Author Affiliations +

Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345394
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We discuss the use of Raman microprobe spectroscopy and Raman imaging to study the chemical composition of fresh, unmounted bone at a microscopic level. A specimen of human cortical bone was analyzed and evidence for the presence of amorphous-type calcium phosphate, a theoretical precursor in the bone formation process, was found. In general the amorphous4ype calcium phosphate appears away from osteons, in the interstitial tissue. This finding calls into question the role of amorphous-type calcium phosphate as a precursor to apatitic phosphate, since it was not found in the recently remodeled bone near the osteon center, but rather in older bone tissue. Some reasons for the presence of amorphous calcium phosphate are proposed. Possible relations ofthe amorphous mineral to bone damage and bone remodeling are discussed.

Citation Download Citation

Angela Carden, Jerilyn A. Timlin, Clare M. Edwards, Michael D. Morris, C. Edward Hoffler, Kenneth Kozloff, and Steven A. Goldstein "Raman imaging of bone mineral and matrix: composition and function", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); https://doi.org/10.1117/12.345394

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