Optical sectioning using finite-sized multiple-aperture arrays in fluorescence direct-view microscopy: experimental results

Patrick J. Smith; Cian M. Taylor; Eithne M. McCabe

doi:10.1117/12.347569

6 May 1999 Optical sectioning using finite-sized multiple-aperture arrays in fluorescence direct-view microscopy: experimental results

Patrick J. Smith, Cian M. Taylor, Eithne M. McCabe

Author Affiliations +

Proceedings Volume 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI; (1999) https://doi.org/10.1117/12.347569
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Confocal fluorescence imaging is widely used, particularly for biological applications, and also notably in direct-view microscopes. Recent work has compared the use of coherent and incoherent illumination sources on the optical sectioning characteristics of fluorescence direct-view microscopes. However this detailed comparison has been done in theory. This paper addresses the experimental aspects of using coherent light sources in fluorescence imaging using a range of finite- sized, multiple-aperture arrays. The experimental difficulties of choosing a suitable uniform, flat, fluorescent plane with a high quantum efficiency are considered. Axial response curves obtained with a fluorescent laser dye sample are presented.

Citation Download Citation

Patrick J. Smith, Cian M. Taylor, and Eithne M. McCabe "Optical sectioning using finite-sized multiple-aperture arrays in fluorescence direct-view microscopy: experimental results", Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); https://doi.org/10.1117/12.347569

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