Application of scanning acoustic and scanning probe microscopy on biological materials to assess elastomechanical properties

Hans-Joachim Hein; D. Matern

doi:10.1117/12.330497

11 November 1998 Application of scanning acoustic and scanning probe microscopy on biological materials to assess elastomechanical properties

Hans-Joachim Hein, D. Matern

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Proceedings Volume 3581, Acousto-Optics and Applications III; (1998) https://doi.org/10.1117/12.330497
Event: Acousto-Optics and Applications III, 1998, Gdansk-Jurata, Poland

Abstract

The principles of scanning acoustic microscope (SAM) and the scanning probe microscope (SPM) are presented. Especially the modes for application on biological specimen are described. We focused our attention on the possibilities to determine the elastomechanical properties of tissues. For bone structures the V(z)-behavior is used to determine tissue properties. From this the sound propagating velocity has been calculated to assess the modulus of elasticity. We applied this method on cortical bone and to assess the mineralization at the callus tissue after distraction. In similar manner the SPM as completing techniques is applied. For this method we used the so-called 'tapping mode' for diminishing the influence of scanning procedure on the surface of specimen. It has been shown that the apatite crystals are imaged as light areas and the more soft collagen as darker regions. On this way the dimensions of crystals and the thickness of collagen fibers between them could be measured. The angle dependency of elastomechanical properties could be shown by cutting the bone samples under different angles and by measurement of sound propagating velocities into these specimens as well their imaging by SPM. The results are discussed and related to clinical findings.

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Hans-Joachim Hein and D. Matern "Application of scanning acoustic and scanning probe microscopy on biological materials to assess elastomechanical properties", Proc. SPIE 3581, Acousto-Optics and Applications III, (11 November 1998); https://doi.org/10.1117/12.330497

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KEYWORDS

Bone

Acoustics

Crystals

Scanning probe microscopy

Tissues

Apatite

Wave propagation

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