Optochemical nanosensors for intracellular chemical measurement

Raoul Kopelman; Michael T. Miller; Murphy Brasuel; Heather A. Clark; Marion Hoyer; Martin A. Philbert

doi:10.1117/12.339797

23 February 1999 Optochemical nanosensors for intracellular chemical measurement

Raoul Kopelman, Michael T. Miller, Murphy Brasuel, Heather A. Clark, Marion Hoyer, Martin A. Philbert

Proceedings Volume 3540, Chemical, Biochemical, and Environmental Fiber Sensors X; (1999) https://doi.org/10.1117/12.339797
Event: Photonics East (ISAM, VVDC, IEMB), 1998, Boston, MA, United States

Abstract

The development of a submicron optical fiber `supertip' has provided advantages over previously produced submicron tips, such as facilitating insertion of these sensors into cells while minimizing damage to the cell membrane. Fiber optic ion correlation-based nanosensors for sodium, potassium and chloride employing these `supertips' have been applied to the monitoring of ion concentrations in single mouse oocytes. These sensors have also been used to monitor the effect of an ion channel-blocking agent. In order to address the challenge associated with single-cell simultaneous measurement of multiple analytes, the use of submicron optical fiber multiprobes has been explored.

Citation Download Citation

Raoul Kopelman, Michael T. Miller, Murphy Brasuel, Heather A. Clark, Marion Hoyer, and Martin A. Philbert "Optochemical nanosensors for intracellular chemical measurement", Proc. SPIE 3540, Chemical, Biochemical, and Environmental Fiber Sensors X, (23 February 1999); https://doi.org/10.1117/12.339797

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