Concentration, size, and excitation power effects on fluorescence from microdroplets and microparticles containing tryptophan and bacteria

Nicholas F. Fell Jr.; Ronald G. Pinnick; Steven C. Hill; Gorden W. Videen; Stanley Niles; Richard K. Chang; Stephen Holler; Yongle Pan; Jerold R. Bottiger; Burt V. Bronk

doi:10.1117/12.336843

18 January 1999 Concentration, size, and excitation power effects on fluorescence from microdroplets and microparticles containing tryptophan and bacteria

Nicholas F. Fell Jr., Ronald G. Pinnick, Steven C. Hill, Gorden W. Videen, Stanley Niles, Richard K. Chang, Stephen Holler, Yongle Pan, Jerold R. Bottiger, Burt V. Bronk

Author Affiliations +

Proceedings Volume 3533, Air Monitoring and Detection of Chemical and Biological Agents; (1999) https://doi.org/10.1117/12.336843
Event: Photonics East (ISAM, VVDC, IEMB), 1998, Boston, MA, United States

Abstract

Our group has been developing a system for single-particle fluorescence detection of aerosolized agents. This paper describes the most recent steps in the evolution of this system. The effects of fluorophore concentrations, droplet size, and excitation power have also been investigated with microdroplets containing tryptophan in water to determine the effects of these parameters on our previous results. The vibrating orifice droplet generator was chosen for this study base don its ability to generate particles of well- known and reproducible size. The power levels required to reach saturation and photodegradation were determined. In addition, the collection of fluorescence emission was optimized through the use of a UV achromatic photographic lens. This arrangement permitted collection of images of the droplet stream. Finally, the use of a dual-beam, conditional firing scheme facilitated the collection of improved signal- to-noise single-shot spectra from individual biological particles.

Citation Download Citation

Nicholas F. Fell Jr., Ronald G. Pinnick, Steven C. Hill, Gorden W. Videen, Stanley Niles, Richard K. Chang, Stephen Holler, Yongle Pan, Jerold R. Bottiger, and Burt V. Bronk "Concentration, size, and excitation power effects on fluorescence from microdroplets and microparticles containing tryptophan and bacteria", Proc. SPIE 3533, Air Monitoring and Detection of Chemical and Biological Agents, (18 January 1999); https://doi.org/10.1117/12.336843

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