Biological transport in a microfabricated device: active immunochromatography with motorized antibodies

Loren Limberis; Russell J. Stewart

doi:10.1117/12.322097

10 September 1998 Biological transport in a microfabricated device: active immunochromatography with motorized antibodies

Loren Limberis, Russell J. Stewart

Author Affiliations +

Proceedings Volume 3515, Microfluidic Devices and Systems; (1998) https://doi.org/10.1117/12.322097
Event: Micromachining and Microfabrication, 1998, Santa Clara, CA, United States

Abstract

Kinesin motor proteins transduce chemical energy released by adenosine triphosphate hydrolysis into mechanical force and motion along microtubule tracks. Biological cells use kinesin motors to transport intracellular components to specific cellular locations. Our goal is to mimic some aspects of kinesin's cellular function and apply them to an active separation microdevice. Here we report the coupling of cargo-binding domains to recombinant kinesin. Metal- chelating Pluronic surfactants were used to specifically co- immobilize recombinant kinesin motor proteins and two immunoglobulin-(IgG) binding domains of protein A on the surface of polystyrene beads. Monoclonal antibodies, the cargo-binding domains, were indirectly coupled to kinesin on the beads through the IgG-binding domains. These motorized antibodies are capable of specifically binding virtually any cargo (antigens) and transporting them along microtubule tracks to different locations or compartments in an active separation microdevice.

Citation Download Citation

Loren Limberis and Russell J. Stewart "Biological transport in a microfabricated device: active immunochromatography with motorized antibodies", Proc. SPIE 3515, Microfluidic Devices and Systems, (10 September 1998); https://doi.org/10.1117/12.322097

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available