PDT mechanism of phthalocyanines by ultrastructural cytochemical cerium deposition localization EDX technique and apoptosis detection

Hong-Yu Yu; Yong-Ji Yang; Ji-Hong Sha; Rong-Chun Dong; Cheng-Zhi Xiao; Fa-Pu Chen

doi:10.1117/12.330164

2 November 1998 PDT mechanism of phthalocyanines by ultrastructural cytochemical cerium deposition localization EDX technique and apoptosis detection

Hong-Yu Yu, Yong-Ji Yang, Ji-Hong Sha, Rong-Chun Dong, Cheng-Zhi Xiao, Fa-Pu Chen

Author Affiliations +

Proceedings Volume 3344, 1997 Shanghai International Conference on Laser Medicine and Surgery; (1998) https://doi.org/10.1117/12.330164
Event: 1997 Shanghai International Conference on Laser Medicine and Surgery, 1997, Shanghai, China

Abstract

Both the chemical mechanisms of photodynamic therapy (PDT) with new photosensitizer phthalocyanine (PC) and the relationship between the chemical mechanisms and the pathologic mechanism in PDT with PC (PC PDT) are not exactly clear. In this study, the mechanisms of PC-PDT in vitro and in vivo were aimed with research methods of ultrastructural cytochemical cerium deposition locations, EDX technique and apoptosis detection. Three kinds of PCs, disulfonated chloroaluminium PC, AlS₃PC and disulfonated zinc PC, were adopted in the experiments. The result revealed: (1) There are a direct proportion between the amount of the ¹O₂ enhancer and the amount of cerium deposition and an inverse ratio between the amount of the ¹O₂ quencher and the deposition. So the dominant mechanism of PC-PDT is ¹O₂; (2) The ultrastructural sties of most obvious pathologic damage ar the sites of richness in PC and the sites of richness in cerium deposition; the mitochondria is one of the most important PDT targets in tumor cells, and secondly other cytomembrane structures are also important sites injured by PC-PDT; both the PDT damages to tumor cells and that to microvasculature are the significant pathologic mechanisms for killing tumor cells in vivo; (3) Apoptosis is an event in the tumor killing process of PC-PDT.

Citation Download Citation

Hong-Yu Yu, Yong-Ji Yang, Ji-Hong Sha, Rong-Chun Dong, Cheng-Zhi Xiao, and Fa-Pu Chen "PDT mechanism of phthalocyanines by ultrastructural cytochemical cerium deposition localization EDX technique and apoptosis detection", Proc. SPIE 3344, 1997 Shanghai International Conference on Laser Medicine and Surgery, (2 November 1998); https://doi.org/10.1117/12.330164

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