Vibrational echo studies of proteins, liquids, and glasses

Kirk D. Rector; Michael D. Fayer

doi:10.1117/12.306138

24 April 1998 Vibrational echo studies of proteins, liquids, and glasses

Kirk D. Rector, Michael D. Fayer

Proceedings Volume 3273, Laser Techniques for Condensed-Phase and Biological Systems; (1998) https://doi.org/10.1117/12.306138
Event: Optoelectronics and High-Power Lasers and Applications, 1998, San Jose, CA, United States

Abstract

Ultrafast IR vibrational echo experiments, which are used to examine liquids, glasses, and proteins are described. Like the NMR spin echo and other NMR pulse sequences, the vibrational echo can extract dynamical and spectroscopic information that cannot be obtained from a vibrational absorption spectrum. The vibrational echo measures the homogeneous vibrational linewidth even if the absorption line is massively inhomogeneously broadened. When combined with pump-probe experiments, the homogeneous pure dephasing is obtained. Conducting these experiments as a function of temperature provides information in dynamics and intermolecular interactions. The nature of the method and the experimental procedures are outlined. Experimental results are presented for the metal carbonyl solute, Rh(CO)₂acac, in liquid and glassy dibutyl phthalate. The dynamics of the CO ligand bound at the active site of the protein myoglobin is also examined and compared with that in several myoglobin mutants. The results provide insights into protein dynamics and how protein structural fluctuations are communicated to a ligand bound at the active site.

Citation Download Citation

Kirk D. Rector and Michael D. Fayer "Vibrational echo studies of proteins, liquids, and glasses", Proc. SPIE 3273, Laser Techniques for Condensed-Phase and Biological Systems, (24 April 1998); https://doi.org/10.1117/12.306138

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