Spectroscopic investigation of the nicotinic acetylcholine receptor for application in medical diagnosis

Reiner Salzer; Wolfgang B. Fischer; Ines Unverricht; Dirk Schwenke; Gerald Steiner; Andre Schrattenholz; Alfred Maelicke

doi:10.1117/12.306096

24 April 1998 Spectroscopic investigation of the nicotinic acetylcholine receptor for application in medical diagnosis

Reiner Salzer, Wolfgang B. Fischer, Ines Unverricht, Dirk Schwenke, Gerald Steiner, Andre Schrattenholz, Alfred Maelicke

Author Affiliations +

Proceedings Volume 3257, Infrared Spectroscopy: New Tool in Medicine; (1998) https://doi.org/10.1117/12.306096
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Native vesicles containing the nicotinic acetylcholine receptor (nAChR) prepared from the electric organ of the ray Torpedo marmorata were used to obtain fluorescence signal sin dependence of different concentrations of the local anesthetics procaine. Nonlinear concentration dependent spectral changes are found using ethidium bromide as a fluorescence marker. Structural changes are found for the proteins including the nAChR in the vesicles during immobilization onto surfaces such as IR transparent germanium (GE) crystal, Ge crystal coated with silver (Ag) cluster to use the SEIRA effect and/or crystals covered with a lipid subphase. A comparison between Ge and Ge coated with Ag (Ge/Ag) clusters reveals increased structural changes in the spectral regions around 1670 cm^-1 upon adsorption of the vesicles on the latter surface. Is the Ge/Ag crystal precoated with a lipid subphase an almost similar spectral contour for the amide I band envelope as in the spectra recorded on a neat Ge crystal is found.

Citation Download Citation

Reiner Salzer, Wolfgang B. Fischer, Ines Unverricht, Dirk Schwenke, Gerald Steiner, Andre Schrattenholz, and Alfred Maelicke "Spectroscopic investigation of the nicotinic acetylcholine receptor for application in medical diagnosis", Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); https://doi.org/10.1117/12.306096

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