Human cornea wound healing in organ culture after Er:YAG laser ablation

Jin-Hui Shen; Karen Margaret Joos M.D.; Richard D. Robinson; Debra J. Shetlar; Denis M. O'Day M.D.

doi:10.1117/12.309425

1 June 1998 Human cornea wound healing in organ culture after Er:YAG laser ablation

Jin-Hui Shen, Karen Margaret Joos M.D., Richard D. Robinson, Debra J. Shetlar, Denis M. O'Day M.D.

Proceedings Volume 3246, Ophthalmic Technologies VIII; (1998) https://doi.org/10.1117/12.309425
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Purpose: To study the healing process in cultured human corneas after Er:YAG laser ablation. Methods: Human cadaver corneas within 24 hours post mortem were ablated with a Q- switched Er:YAG laser at 2.94 micrometer wavelength. The radiant exposure was 500 mJ/cm². The cornea was cultured on a tissue supporting frame immediately after the ablation. Culture media consisted of 92% minimum essential media, 8% fetal bovine serum, 0.125% HEPES buffer solution, 0.125% gentamicin, and 0.05% fungizone. The entire tissue frame and media container were kept in an incubator at 37 degrees Celsius and 5% CO₂. Serial macroscopic photographs of the cultured corneas were taken during the healing process. Histology was performed after 30 days of culture. Results: A clear ablated crater into the stroma was observed immediately after the ablation. The thickness of thermal damage ranges between 1 and 25 micrometer. Haze development within the crater varies from the third day to the fourteenth day according to the depth and the roughness of the crater. Histologic sections of the cultured cornea showed complete re- epithelization of the lased area. Loose fibrous tissue is observed filling the ablated space beneath the epithelium. The endothelium appeared unaffected. Conclusions: The intensity and time of haze development appears dependent upon the depth of the ablation. Cultured human corneas may provide useful information regarding the healing process following laser ablation.

Citation Download Citation

Jin-Hui Shen, Karen Margaret Joos M.D., Richard D. Robinson, Debra J. Shetlar, and Denis M. O'Day M.D. "Human cornea wound healing in organ culture after Er:YAG laser ablation", Proc. SPIE 3246, Ophthalmic Technologies VIII, (1 June 1998); https://doi.org/10.1117/12.309425

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