Use of the polarization vector in modeling tissue fluorescence: theoretical and experimental comparison

Ourania Panou-Diamanti; Nikolaos K. Uzunoglou; A. Vasiliou; Giannis Zacharakis; George Filippidis; Theodore G. Papazoglou; Dimitrios Koutsouris

doi:10.1117/12.297953

29 December 1997 Use of the polarization vector in modeling tissue fluorescence: theoretical and experimental comparison

Ourania Panou-Diamanti, Nikolaos K. Uzunoglou, A. Vasiliou, Giannis Zacharakis, George Filippidis, Theodore G. Papazoglou, Dimitrios Koutsouris

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Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297953
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

This paper describes an innovative approach to tissue fluorescence as a phenomenon of inelastic scattering of electromagnetic radiation. A general formulation of the polarization vector of the medium is used in order to describe the fluorescence inelastic scattering process. The general method described, is applied to two different tissue models. In the first model, tissue is represented as a single infinitely thick, homogeneous layer, under plane wave excitation. The second model used is based on a picture of tissue as a single dielectric layer, under pulse excitation. In both cases, fluorophores and absorbing species are assumed to be homogeneously distributed inside tissue. The mathematical techniques used together with the fully evaluated theoretical results are presented. Additionally, experimental measurements were performed on collagen gels which contained various dyes in order to evaluate and validate our mathematical modeling.

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Ourania Panou-Diamanti, Nikolaos K. Uzunoglou, A. Vasiliou, Giannis Zacharakis, George Filippidis, Theodore G. Papazoglou, and Dimitrios Koutsouris "Use of the polarization vector in modeling tissue fluorescence: theoretical and experimental comparison", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297953

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KEYWORDS

Tissues

Luminescence

Mathematical modeling

Scattering

Electromagnetic scattering

Dielectrics

Data modeling

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