Paper
29 December 1997 Multiple fluorescence microscopy and optoelectronic imaging: possibilities and limits
Heinz Gundlach
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Abstract
The last 20 years have seen an unexpected great renaissance and a partial revolution in light microscopy. This recent progress is due to new design in optics and instrumentation as well as improvement of optical contrast enhancement techniques. Recent progress in fluorescence microscopy is achieved by multiparameter fluorescence techniques, by improvement of conventional photomicrography as well as by optoelectronic imaging, confocal laser scanning microscopy, image processing and analysis. Due to the increase in number of fluorescence dyes, double and triple bandpass filter sets permit a rapid changeover between different fluorochromes simultaneously.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Heinz Gundlach "Multiple fluorescence microscopy and optoelectronic imaging: possibilities and limits", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297968
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KEYWORDS
Luminescence

Microscopy

Electronic filtering

Linear filtering

Bandpass filters

Digital imaging

Optoelectronics

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