Effect of liquid nitrogen and formalin-based conservation in the in-vitro measurement of laser-induced fluorescence of peripheral vascular tissue

George Filippidis; Giannis Zacharakis; A. Katsamouris; M. Kouktzela; Sune Montan; Stefan Andersson-Engels; Theodore G. Papazoglou

doi:10.1117/12.297984

29 December 1997 Effect of liquid nitrogen and formalin-based conservation in the in-vitro measurement of laser-induced fluorescence of peripheral vascular tissue

George Filippidis, Giannis Zacharakis, A. Katsamouris, M. Kouktzela, Sune Montan, Stefan Andersson-Engels, Theodore G. Papazoglou

Author Affiliations +

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297984
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

In order to investigate the effects of conservation in liquid nitrogen and formalin on peripheral vascular tissue (abdominal aortic, femoral and flank artery tissue) laser-induced fluorescence spectra were recorded during the exposure of these tissues to He-Cd radiation (442 nm). The spectral distribution of tissue fluorescence allowed the development of simple algorithms based on the intensity difference in order to discriminate the tissue samples when they were fresh and after they were stored for 24 and 48 hours in liquid nitrogen or formalin.

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George Filippidis, Giannis Zacharakis, A. Katsamouris, M. Kouktzela, Sune Montan, Stefan Andersson-Engels, and Theodore G. Papazoglou "Effect of liquid nitrogen and formalin-based conservation in the in-vitro measurement of laser-induced fluorescence of peripheral vascular tissue", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297984

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